Exploring Distinct Second-Order Data Approaches for Thiamine Quantification via Carbon Dot/Silver Nanoparticle FRET Reversion

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Castro, RC (Author) Other; Páscoa, RNMJ (Author) Other; Lucia L M F S Saraiva (Author) FFUP; Joao L M Santos (Author) FFUP; Ribeiro, DSM (Author) Other

Journal

Title: Biosensors-Basel

Vol. 14

Final page: 604

ISSN: 2079-6374

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-017-MP4

DOI: 10.3390/bios14120604

Abstract (EN): Accurate and selective monitoring of thiamine levels in multivitamin supplements is essential for preventing deficiencies and ensuring product quality. To achieve this, a F & ouml;rster resonance energy transfer (FRET) system using carbon dots (CDs) as energy donors and citrate-stabilized silver nanoparticles (AgNPs) as energy acceptors was developed. The aqueous synthesis of AgNPs using microwave irradiation was optimized to obtain efficient plasmonic nanoparticles for FRET applications, targeting maximal absorbance intensity, stability, and wavelength alignment. Using a central composite orthogonal design (CCOD), the optimal conditions were identified as a 12.5 min microwave reaction time, a Ag molar ratio of 0.72, and a pH of 8.28. The FRET sensing scheme was applied for thiamine determination, where the vitamin's presence impaired the FRET process, restoring CDs' photoluminescence (PL) emission in a concentration-dependent manner. To mitigate interference from other vitamins, PL kinetic data and excitation-emission matrix (EEM) data were analyzed using unfolded partial least-squares (U-PLS) with the subsequent application of the residual bilinearization technique (RBL), achieving high sensitivity and specificity for thiamine detection. This method demonstrated its accuracy and robustness by attaining a determination coefficient (R2) of 0.952 and a relative error of prediction (REP%) of 11%. This novel method offers highly sensitive and interference-free thiamine detection, with significant potential for a wide range of analytical applications.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 20