Cancer Exosomes Perform Cell-Independent MicroRNA Biogenesis and Promote Tumorigenesis

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Melo, SA (Author) Other; Sugimoto, H (Author) Other; O'Connell, JT (Author) Other; Kato, N (Author) Other; Villanueva, A (Author) Other; Vidal, A (Author) Other; Qiu, L (Author) Other; Vitkin, E (Author) Other; Perelman, LT (Author) Other; Melo, CA (Author) Other; Lucci, A (Author) Other; Ivan, C (Author) Other; Calin, GA (Author) Other; Kalluri, R (Author) Other

Journal

Title: Cancer Cell

Vol. 26

Pages: 707-721

ISSN: 1535-6108

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 992 Citations

Scopus - 1032 Citations

Other information

Authenticus ID: P-00A-077

DOI: 10.1016/j.ccell.2014.09.005

Abstract (EN): Exosomes are secreted by all cell types and contain proteins and nucleic acids. Here, we report that breast cancer associated exosomes contain microRNAs (miRNAs) associated with the RISC-Loading Complex (RLC) and display cell-independent capacity to process precursor microRNAs (pre-miRNAs) into mature miRNAs. Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer cells. CD43 mediates the accumulation of Dicer specifically in cancer exosomes. Cancer exosonnes mediate an efficient and rapid silencing of mRNAs to reprogram the target cell transcriptome. Exosomes derived from cells and sera of patients with breast cancer instigate nontumorigenic epithelial cells to form tumors in a Dicer-dependent manner. These findings offer opportunities for the development of exosomes based biomarkers and therapies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15