The LINC complex ensures accurate centrosome positioning during prophase

• Type: Article in International Scientific Journal
• Date: 2024-01-16

Authors

J Ferreira (Author) FMUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: LIFE SCIENCE ALLIANCE

Vol. 7 No. 4

ISSN: 2575-1077

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ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-00Z-TQF

DOI: 10.26508/lsa.202302404

Abstract (EN): Accurate centrosome separation and positioning during early mitosis relies on force-generating mechanisms regulated by a combination of extracellular, cytoplasmic, and nuclear cues. The identity of the nuclear cues involved in this process remains largely unknown. Here, we investigate how the prophase nucleus contributes to centrosome positioning during the initial stages of mitosis, using a combination of cell micropatterning, highresolution live-cell imaging, and quantitative 3D cellular reconstruction. We show that in untransformed RPE-1 cells, centrosome positioning is regulated by a nuclear signal, independently of external cues. This nuclear mechanism relies on the linker of nucleoskeleton and cytoskeleton complex that controls the timely loading of dynein on the nuclear envelope (NE), providing spatial cues for robust centrosome positioning on the shortest nuclear axis, before nuclear envelope permeabilization. Our results demonstrate how nuclear-cytoskeletal coupling maintains a robust centrosome positioning mechanism to ensure efficient mitotic spindle assembly.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17