ACE and ACE2 catalytic activity in the fecal content along the gut

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Ferreira Duarte, M (Author) Other; Oliveira, LCG (Author) Other; Clara Quintas (Author) FFUP; Esteves Monteiro, M (Author) Other; Duarte Araujo, M (Author) Other; Sousa, T (Author) FMUP; Casarini, DE (Author) Other; Morato, M (Author) FFUP

Journal

Title: Neurogastroenterology and Motility

Vol. 35

ISSN: 1350-1925

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00Y-A0D

DOI: 10.1111/nmo.14598

Abstract (EN): Background: Angiotensin-converting enzyme (ACE) and ACE2 are two major enzymes of the renin-angiotensin-aldosterone system (RAAS), which control the formation/degradation of angiotensin (Ang) II and Ang1-7, regulating their opposite effects. We aimed at evaluating the catalytic activity of ACE and ACE2 in the intestinal content and corresponding intestinal tissue along the gut of Wistar Han rats. Methods: Portions of the ileum, cecum, proximal colon, and distal colon, and the corresponding intestinal content were collected from Wistar Han rats. Enzyme activity was evaluated by fluorometric assays using different substrates: Hippuryl-His-Leu for ACE-C-domain, Z-Phe-His-Leu for ACE-N-domain, and Mca-APK(Dnp) for ACE2. ACE and ACE2 concentration was assessed by ELISA. Ratios concerning concentrations and activities were calculated to evaluate the balance of the RAAS. Statistical analysis was performed using Friedman test followed by Dunn's multiple comparisons test or Wilcoxon matched-pairs test whenever needed. Key Results: ACE and ACE2 are catalytically active in the intestinal content along the rat gut. The ACE N-domain shows higher activity than the C-domain both in the intestinal content and in the intestinal tissue. ACE and ACE2 are globally more active in the intestinal content than in the corresponding intestinal tissue. There was a distal-to-proximal prevalence of ACE2 over ACE in the intestinal tissue. Conclusions & Inferences: This work is the first to report the presence of catalytically active ACE and ACE2 in the rat intestinal content, supporting future research on the regulatory role of the intestinal RAAS on gut function and a putative link to the microbiome.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10