Process integration of separation of amino acids by a temperature-induced aqueous two-phase system

• Type: Article in International Scientific Journal
• Date: 2002

Authors

Mian Li (Author) Other; Zi-Qiang (Author) Other; Alírio E. Rodrigues (Author) FEUP

Journal

Title: IECR

Vol. 41 No. 2

Pages: 251-256

Indexing

ISI Web of Knowledge - 17 Citations

ISI Web of Science

Scopus - 20 Citations

COMPENDEX

Scientific classification

CORDIS: Technological sciences > Technology > Biotecnology > Downstream processing

Other information

Authenticus ID: P-000-QJY

DOI: 10.1021/ie010060t

Abstract (EN): Random copolymers of ethylene oxide and propylene oxide (EOPO) and (hydroxypropyl)starch (PES) have been used in aqueous two-phase partitioning of amino acids. The partitioning behaviors of L-Ile and L-Val were investigated in EOPO/PES aqueous two-phase systems (ATPSs). The effects of the addition of salt and pH on the partitioning were studied. Fundamental data were employed for further scale-up to process 1000 mL of fermentation broth. An integrated bioseparation process was designed on the basis of the partitioning behavior of amino acids in the primary ATPSs and in the temperature-induced phase systems. L-Ile crystals were finally obtained through (1) selective partition in the EOPO/PES system, (2) recovery from the water phase during the temperature-induced phase separation of the EOPO phase, and (3) crystallization from the water phase by isoelectric precipitation. The mass of L-Ile crystals was 13.76 g with a purity of 97.17%. The overall recovery was 74.28%. Aqueous two-phase extraction combined with temperature-induced phase separation appears to be a promising alternative in the amino acid industry.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5