An integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Ferreira, AM (Author) Other; Passos, H (Author) Other; Okafuji, A (Author) Other; Tavares, APM (Author) Other; Ohno, H (Author) Other; Freire, MG (Author) Other; Coutinho, JAP (Author) Other

Journal

Title: Green Chemistry

Vol. 20

Pages: 1218-1223

ISSN: 1463-9262

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 28 Citations

Other information

Authenticus ID: P-00N-SAT

DOI: 10.1039/c7gc03880a

Abstract (EN): Herein, thermoreversible aqueous biphasic systems (ABS) composed of ammonium-based zwitterions (ZIs) and polymers are disclosed to act as integrated bioreaction-separation processes. The biocatalytic reaction involving laccase occurs in homogeneous media, after which small changes in temperature induce the formation of two phases and the complete separation of the enzyme from the products in a single-step. These systems also allow the recovery and reuse of the enzyme, along with the ZI-rich phase, contributing towards the development of sustainable biocatalytic processes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6