Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Godinho, O (Author) Other; Klimek, D (Author) Other; Jackiewicz, A (Author) Other; Guedes, B (Author) Other; Almeida, E (Author) Other; Calisto, R (Author) Other; Vitorino, IR (Author) Other; Santos, JDN (Author) Other; Gonzalez, I (Author) Other; Lobo-da-Cunha, A (Author) Other; Calusinska, M (Author) Other; Quinteira, S (Author) FCUP; lage, om (Author) FCUP

Journal

Title: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Vol. 116

Pages: 1209-1225

ISSN: 0003-6072

Publisher: Springer Nature

Indexing

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Other information

Authenticus ID: P-00Z-3DP

DOI: 10.1007/s10482-023-01877-2

Abstract (EN): A bacterial strain was isolated from a brackish water sample of Tagus river, Alcochete, Portugal and was designated TO1_6(T). It forms light pink colonies on M13 medium supplemented with N-acetylglucosamine. Cells are pear-shaped to spherical, form rosettes and divide by budding. Strain TO1_6(T) presents a mesophilic and neutrophilic profile, with optimum growth at 20 to 25 degrees C and pH 7.0 to 7.5, and vitamin supplementation is not required to promote its growth. The genome of the novel isolate is 7.77 Mbp in size and has a DNA G+C content of 56.3%. Based on its 16S rRNA gene sequence, this strain is affiliated with the phylum Planctomycetota. Further taxonomic characterization using additional phylogenetic markers, namely rpoB gene sequence (encoding the beta-subunit of the DNA-dependent RNA polymerase), as well as Percentage of conserved proteins, average nucleotide identity and average amino acid identity, suggest the affiliation of strain TO1_6(T) to the genus Stieleria, a recently described taxon in the family Pirellulaceae, order Pirellulales and class Planctomycetia. Based on the genotypic, phylogenetic and physiological characterization, we here describe a new species represented by the type strain TO1_6(T) (=CECT 30432(T), =LMG 32465(T)), for which the name Stieleria tagensis sp. nov. is proposed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17