Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Zhang, HY (Author) Other; Freitas, D (Author) Other; Kim, HS (Author) Other; Fabijanic, K (Author) Other; Li, Z (Author) Other; Chen, HY (Author) Other; Mark, MT (Author) Other; Molina, H (Author) Other; Martin, AB (Author) Other; Bojmar, L (Author) Other; Fang, J (Author) Other; Rampersaud, S (Author) Other; Hoshino, A (Author) Other; Matei, I (Author) Other; Kenific, CM (Author) Other; Nakajima, M (Author) Other; Mutvei, AP (Author) Other; Sansone, P (Author) Other; Buehring, W (Author) Other; Wang, HJ (Author) Other; Jimenez, JP (Author) Other; Cohen Gould, L (Author) Other; Paknejad, N (Author) Other; Brendel, M (Author) Other; Manova Todorova, K (Author) Other; Magalhaes, A (Author) Other; Ferreira, JA (Author) Other; Osorio, H (Author) Other; Silva, AM (Author) Other; Massey, A (Author) Other; Cubillos Ruiz, JR (Author) Other; Galletti, G (Author) Other; Giannakakou, P (Author) Other; Cuervo, AM (Author) Other; Blenis, J (Author) Other; Schwartz, R (Author) Other; Brady, MS (Author) Other; Peinado, H (Author) Other; Bromberg, J (Author) Other; Matsui, H (Author) Other; Celso Reis (Author) ICBAS; Lyden, D (Author) Other

Journal

Title: Nature Cell Biology

Vol. 20

Pages: 332-+

ISSN: 1465-7392

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 966 Citations

Scopus - 993 Citations

Other information

Authenticus ID: P-00N-N17

DOI: 10.1038/s41556-018-0040-4

Abstract (EN): The heterogeneity of exosomal populations has hindered our understanding of their biogenesis, molecular composition, biodistribution and functions. By employing asymmetric flow field-flow fractionation (AF4), we identified two exosome subpopulations (large exosome vesicles, Exo-L, 90-120 nm; small exosome vesicles, Exo-S, 60-80 nm) and discovered an abundant population of non-membranous nanoparticles termed 'exomeres' (similar to 35 nm). Exomere proteomic profiling revealed an enrichment in metabolic enzymes and hypoxia, microtubule and coagulation proteins as well as specific pathways, such as glycolysis and mTOR signalling. Exo-S and Exo-L contained proteins involved in endosomal function and secretion pathways, and mitotic spindle and IL-2/STAT5 signalling pathways, respectively. Exo-S, Exo-L and exomeres each had unique N-glycosylation, protein, lipid, DNA and RNA profiles and biophysical properties. These three nanoparticle subsets demonstrated diverse organ biodistribution patterns, suggesting distinct biological functions. This study demonstrates that AF4 can serve as an improved analytical tool for isolating extracellular vesicles and addressing the complexities of heterogeneous nanoparticle subpopulations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15