Detection of post-translational modifications using solid-phase proximity ligation assay

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Oliveira, FMSD (Author) Other; Mereiter, S (Author) Other; Lönn, P (Author) Other; Siart, B (Author) Other; Shen, Q (Author) Other; Heldin, J (Author) Other; Raykova, D (Author) Other; Karlsson, NG (Author) Other; Polom, K (Author) Other; Roviello, F (Author) Other; Celso Reis (Author) ICBAS; Kamali Moghaddam, M (Author) Other

Journal

Title: New Biotechnology

Vol. 45

Pages: 51-59

ISSN: 1871-6784

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 14 Citations

Other information

Authenticus ID: P-00T-3QF

DOI: 10.1016/j.nbt.2017.10.005

Abstract (EN): Post-translational modifications (PTMs) regulate protein activities to help orchestrate and fine-tune cellular processes. Dysregulation of PTMs is often related with disorders and malignancies, and may serve as a precise biomarker of disease. Developing sensitive tools to measure and monitor low-abundant PTMs in tissue lysates or serum will be instrumental for opening up new PTM-based diagnostic avenues. Here, we investigate the use of solid-phase proximity ligation assay (SP-PLA) for detection of different PTMs. The assay depends on the recognition of the target protein molecule and its modification by three affinity binders. Using antibodies and lectins, we applied the method for detection of glycosylated CD44 and E-Cadherin, and phosphorylated p53 and EGFR. The assay was found to have superior dynamic range and limit of detection compared to standard ELISAs. In summary, we have established the use of SP-PLA as an appropriate method for sensitive detection of PTMs in lysates and sera, which may provide a basis for future PTM-based diagnostic and prognostic biomarkers. © 2017 Elsevier B.V.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9