Identification of novel plasma glycosylation-associated markers of aging

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Catera, M (Author) Other; Borelli, V (Author) Other; Malagolini, N (Author) Other; Chiricolo, M (Author) Other; Venturi, G (Author) Other; Celso Reis (Author) ICBAS; Osorio, H (Author) Other; Abruzzo, PM (Author) Other; Capri, M (Author) Other; Monti, D (Author) Other; Ostan, R (Author) Other; Franceschi, C (Author) Other; Dall'Olio, F (Author) Other

Journal

Title: Oncotarget

Vol. 7

Pages: 7455-7468

ISSN: 1949-2553

Publisher: Impact Journals

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 23 Citations

Other information

Authenticus ID: P-00K-6VD

DOI: 10.18632/oncotarget.7059

Abstract (EN): The pro-or anti-inflammatory activities of immunoglobulins G (IgGs) are controlled by the structure of the glycan N-linked to Asn297 of their heavy chain. The age-associated low grade inflammation (inflammaging) is associated with increased plasmatic levels of agalactosylated IgGs terminating with N-acetylglucosamine (IgG-G0) whose biogenesis has not been fully explained. Although the biosynthesis of glycans is in general mediated by glycosyltransferases associated with internal cell membranes, the extracellular glycosylation of circulating glycoproteins mediated by plasmatic glycosyltransferases has been recently demonstrated. In this study we have investigated the relationship between plasmatic glycosyltransferases, IgG glycosylation and inflammatory and aging markers. In cohorts of individuals ranging from infancy to centenarians we determined the activity of plasmatic beta 4 galactosyltransferase(s) (B4GALTs) and of alpha 2,6-sialyltransferase ST6GAL1, the glycosylation of IgG, the GlycoAge test (a glycosylation-based marker of aging) and the plasma level of inflammatory and liver damage markers. Our results show that: 1) plasmatic B4GALTs activity is a new marker of aging, showing a linear increase throughout the whole age range. 2) plasmatic ST6GAL1 was high only in children and in people above 80, showing a quadratic relationship with age. 3) Neither plasmatic glycosyltransferase correlated with markers of liver damage. 4) plasmatic ST6GAL1 showed a positive association with acute phase proteins in offspring of short lived parents, but not in centenarians or in their offspring. 5) Although the glycosylation of IgGs was not correlated with the level of the two plasmatic glycosyltransferases, it showed progressive age-associated changes consistent with a shift toward a pro-inflammatory glycotype.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14