3D hydrogel mimics of the tumor microenvironment: the interplay among hyaluronic acid, stem cells and cancer cells

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Amorim, S (Author) Other; da Costa, DS (Author) Other; Pashkuleva, I (Author) Other; Celso Reis (Author) ICBAS; Rui Reis (Author) Other; Pires, RA (Author) Other

Journal

Title: Biomaterials Science

Vol. 9

Pages: 252-260

ISSN: 2047-4830

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00T-A5V

DOI: 10.1039/d0bm00843e

Abstract (EN): The present work reports on a 3D model of the tumor microenvironment that contains hyaluronic acid (HA) and alginate, and demonstrates the utility of this model to study the effect of HA size on the crosstalk between cancer cells and mesenchymal stem cells (MSCs). The system incorporates a core that contains HA of specific size (i.e. 6.4, 741 or 1500 kDa) with encapsulated epithelial MKN45 cancer cells and a shell with MSCs that mimic the presence of stem cells next to the tumor site. It was found that short HA (i.e. 6.4 kDa) promotes the invasion of cancer cells from the core to the shell, whereas longer HA (i.e. 741 and 1500 kDa) recruits the MSCs into the core, i.e. the tumor site, where a reduction of the formation of cancer cell aggregates was observed. In summary, the developed 3D model recapitulates some key tumor features related to the effect of HA size on both cancer cell invasiveness and MSC behavior at the tumor site.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9