Studying T cells N -glycosylation by imaging flow cytometry

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Dias, AM (Author) Other; Almeida, CR (Author) Other; Celso Reis (Author) ICBAS; Pinho, SS (Author) Other

Indexing

Scopus - 4 Citations

Other information

Authenticus ID: P-00T-3QD

DOI: 10.1007/978-1-4939-3302-0_11

Abstract (EN): Imaging flow cytometry is an emerging imaging technology that combines features of both conventional flow cytometry and fluorescence microscopy allowing quantification of the imaging parameters. The analysis of protein posttranslational modifications by glycosylation using imaging flow cytometry constitutes an important bioimaging tool in the glycobiology field. This technique allows quantification of the glycan fluorescence intensity, co-localization with proteins, and evaluation of the membrane/cytoplasmic expression. In this chapter we provide the guidelines to analyze glycan expression, particularly the ß1,6 GlcNAc branched N-glycans, on the membrane of intestinal T cells from inflammatory bowel disease patients. © Springer Science+Business Media New York 2016.

Language: English

Type (Professor's evaluation): Scientific