Impact of Truncated O-glycans in Gastric-Cancer-Associated CD44v9 Detection

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Moreira, IB (Author) Other; Pinto, F (Author) Other; Gomes, C (Author) Other; Campos, D (Author) Other; Celso Reis (Author) ICBAS

Journal

Title: Cells

Vol. 9

Final page: 264

ISSN: 2073-4409

Publisher: MDPI

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00R-Z1E

DOI: 10.3390/cells9020264

Abstract (EN): CD44 variant isoforms are often upregulated in cancer and associated with increased aggressive tumor phenotypes. The CD44v9 is one of the major protein splice variant isoforms expressed in human gastrointestinal cancer cells. Immunodetection of CD44 isoforms like CD44v9 in tumor tissue is almost exclusively performed by using specific monoclonal antibodies. However, the structural variability conferred by both the alternative splicing and CD44 protein glycosylation is disregarded. In the present work, we have evaluated the role of O-glycosylation using glycoengineered gastric cancer models in the detection of CD44v9 by monoclonal antibodies. We demonstrated, using different technical approaches, that the presence of immature O-glycan structures, such as Tn and STn, enhance CD44v9 protein detection. These findings can have significant implications in clinical applications mainly at the detection and targeting of this cancer-related CD44v9 isoform and highlight the utmost importance of considering glycan structures in cancer biomarker detection and in therapy targeting.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14