The Preservation of PPAR¿ Genome Duplicates in Some Teleost Lineages: Insights into Lipid Metabolism and Xenobiotic Exploitation

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Páscoa, I (Author) Other; Fonseca, E (Author) Other; Ferraz, R (Author) Other; Machado, AM (Author) Other; Conrado, F (Author) Other; Ruivo, R (Author) Other; Cunha, I (Author) Other; Filipe F C Castro (Author) FCUP

Journal

Title: Genes

Vol. 13

Final page: 107

ISSN: 2073-4425

Publisher: MDPI

Indexing

Scopus - 4 Citations

Other information

Authenticus ID: P-00W-2A9

DOI: 10.3390/genes13010107

Abstract (EN): Three peroxisome proliferator-activated receptor paralogues (PPAR¿,-ß and-¿) are currently recognized in vertebrate genomes. PPAR¿ is known to modulate nutrition, adipogenesis and immunity in vertebrates. Natural ligands of PPAR¿ have been proposed; however, the receptor also binds synthetic ligands such as endocrine disruptors. Two paralogues of PPAR¿ and PPARß have been documented in teleost species, a consequence of the 3R WGD. Recently, two PPAR¿ paralogue genes were also identified in Astyanax mexicanus. We aimed to determine whether the presence of two PPAR¿ paralogues is prevalent in other teleost genomes, through genomic and phylogenetic analysis. Our results showed that besides Characiformes, two PPAR¿ paralogous genes were also identified in other teleost taxa, coinciding with the teleost-specific, whole-genome duplication and with the retention of both genes prior to the separation of the Clupeocephala. To functionally characterize these genes, we used the European sardine (Sardina pilchardus) as a model. PPAR¿A and PPAR¿B display a different tissue distribution, despite the similarity of their functional profiles: they are unresponsive to tested fatty acids and other human PPAR¿ ligands yet yield a transcriptional response in the presence of tributyltin (TBT). This observation puts forward the relevance of comparative analysis to decipher alternative binding architectures and broadens the disruptive potential of man-made chemicals for aquatic species. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Language: English

Type (Professor's evaluation): Scientific