Cadmium effects on net N2O production by the deep-sea isolate Shewanella loihica PV-4

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Pizarro, L (Author) Other; Magalhaes, C (Author) FCUP; Marisa M R Almeida (Author) FCUP; Carvalho, MD (Author) Other; Semedo, M (Author) Other

Journal

Title: FEMS Microbiology Letters

Vol. 370

ISSN: 0378-1097

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00Y-QZG

DOI: 10.1093/femsle/fnad047

Abstract (EN): Deep-sea mining may lead to the release of high concentrations of metals into the surrounding seabed, which can disturb important ecosystem functions provided by microbial communities. Among these, the production of N2O and its reduction to N-2 is of great relevance since N2O is an important greenhouse gas. Metal impacts on net N2O production by deep-sea bacteria are, however, currently unexplored. Here, we evaluated the effects of cadmium (Cd) on net N2O production by a deep-sea isolate, Shewanella loihica PV-4. We performed a series of Cd exposure incubations in oxic conditions and determined N2O fluxes during induced anoxic conditions, as well as the relative expression of the nitrite reductase gene (nirK), preceding N2O production, and N2O reductase gene (nosZ), responsible for N2O reduction. Net N2O production by S. loihica PV-4 exposed to Cd was strongly inhibited when compared to the control treatment (no metal). Both nirK and nosZ gene expression were inhibited in reactors with Cd, but nirK inhibition was stronger, supporting the lower net N2O production observed with Cd. The Cd inhibition of net N2O production observed in this study poses the question whether other deep-sea bacteria would undergo the same effects. Future studies should address this question as well as its applicability to complex communities and other physicochemical conditions, which remain to be evaluated. Cadmium impacts nitrous oxide production in a deep-sea bacterium.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8