Optimized protocol for live-cell analysis of kinetochore fiber maturation in Indian muntjac cells

• Type: Another Publication in an International Scientific Journal
• Date: 2023

Authors

Almeida, AC (Author) Other; Soares de Oliveira, J (Author) Other; Helder Maiato (Author) FMUP

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Authenticus ID: P-00X-ZJ5

DOI: 10.1016/j.xpro.2022.102011

Abstract (EN): Here, we take advantage of the low chromosome number (2N=6) and distinctively large kinetochores of female Indian muntjac cells to investigate the molecular mechanism underlying k-fiber maturation. We describe steps for monitoring kinetochore-microtubule dynamics over time. Specifically, we detail the combination of live-cell super-resolution CH-STED microscopy of microtubule growth events within individual k-fibers and a laser-mediated k-fiber injury/repair assay. These tools provide a direct assessment of microtubule amplification mechanisms within k-fibers in metazoans. For complete details on the use and execution of this protocol, please refer to Almeida et al. (2022).1

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18