Fluorescence in situ hybridization of old G-banded and mounted chromosome preparations

• Type: Article in International Scientific Journal
• Date: 1997

Authors

Gerdes, AM (Author) Other; Pandis, N (Author) Other; Bomme, L (Author) Other; Dietrich, CU (Author) Other; Manuel R Teixeira (Author) Other; Bardi, G (Author) Other; Heim, S (Author) Other

Journal

Title: Cancer Genetics and Cytogenetics

Vol. 98

Pages: 9-15

ISSN: 0165-4608

Publisher: Elsevier B.V.

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-008-QA5

DOI: 10.1016/s0165-4608(96)00407-4

Abstract (EN): An improved method for fluorescence in situ hybridization (FISH) investigation of old, previously G-banded, mounted chromosome preparations with chromosome specific painting probes and centromere-specific probes is described. Before hybridization, the slides are incubated in xylene until the coverslips detach spontaneously; any mechanical manipulation will jeopardize the results. The success of chromosome painting is improved by excluding the regular RNase treatment step prior to hybridization. Additional changes compared with standard FISH protocols are that the 2 x SSC step is omitted, that the amount of added probe is increased approximately 2.5 times, and that the amplification of signals is performed twice. The applicability of the method, which allows double painting with two differently labeled probes using two differently fluorescing colors, was tested on 11 cases involving different chromosome abnormalities and different types of material, including short-term cultures of epithelial and mesenchymal tumors, blood, leukemic bone marrow, and long-term cultures of a cell line derived from an epithelial tumor. Success was achieved even with chromosome preparations that were several years old. (C) Elsevier Science Inc., 1997.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7