Novel 5 ' Fusion Partners of ETV1 and ETV4 in Prostate Cancer

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Barros Silva, JD (Author) Other; Paulo, P (Author) Other; Bakken, AC (Author) Other; cerveira, n (Author) Other; Lovf, M (Author) Other; henrique, rmf (Author) ICBAS; Carmen Jeronimo (Author) ICBAS; Lothe, RA (Author) Other; Skotheim, RI (Author) Other; Manuel R Teixeira (Author) ICBAS

Journal

Title: Neoplasia

Vol. 15

Pages: 720-+

ISSN: 1522-8002

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 28 Citations

Other information

Authenticus ID: P-006-9AM

DOI: 10.1593/neo.13232

Abstract (EN): Gene fusions involving the erythroblast transformation-specific (ETS) transcription factors ERG, ETV1, ETV4, ETV5, and FLI1 are a common feature of prostate carcinomas (PCas). The most common upstream fusion partner described is the androgen-regulated prostate-specific gene TMPRSS2, most frequently with ERG, but additional 5' fusion partners have been described. We performed 5' rapid amplification of cDNA ends in 18 PCas with ETV1, ETV4, or ETV5 outlier expression to identify the 5' fusion partners. We also evaluated the exon-level expression profile of these ETS genes in 14 cases. We identified and confirmed by fluorescent in situ hybridization (FISH) and reverse transcription-polymerase chain reaction the two novel chimeric genes OR51E2-ETV1 and UBTF-ETV4 in two PCas. OR51E2 encodes a G-protein-coupled receptor that is overexpressed in PCas, whereas UBTF is a ubiquitously expressed gene encoding an HMG-box DNA-binding protein involved in ribosome biogenesis. We additionally describe two novel gene fusion combinations of previously described genes, namely, SLC45A3-ETV4 and HERVK17-ETV4. Finally, we found one PCa with TMPRSS2-ETV1, one with C15orf21-ETV1, one with EST14-ETV1, and two with 14q133-q21.1-ETV1. In nine PCas (eight ETV1 and one ETV5), exhibiting ETS outlier expression and genomic rearrangement detected by FISH, no 5' fusion partner was found. Our findings contribute significantly to characterize the heterogeneous group of ETS gene fusions and indicate that all genes described as 5' fusion partners with one ETS gene can most likely be rearranged with any of the other ETS genes involved in prostate carcinogenesis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9