DNA repair genes are selectively mutated in diffuse large B cell lymphomas

• Type: Article in International Scientific Journal
• Date: 2013

Authors

de Miranda, NFCC (Author) Other; Peng, RJ (Author) Other; Georgiou, K (Author) Other; Wu, CL (Author) Other; Sorqvist, EF (Author) Other; Berglund, M (Author) Other; Chen, LY (Author) Other; Gao, ZB (Author) Other; Lagerstedt, K (Author) Other; Lisboa, S (Author) Other; Roos, F (Author) Other; van Wezel, T (Author) Other; Manuel R Teixeira (Author) ICBAS; Rosenquist, R (Author) Other; Sundstrom, C (Author) Other; Enblad, G (Author) Other; Nilsson, M (Author) Other; Zeng, YX (Author) Other; Kipling, D (Author) Other; Pan Hammarstrom, Q (Author) Other

Journal

Title: Journal of Experimental Medicine

Vol. 210

Pages: 1729-1742

ISSN: 0022-1007

Publisher: Rockefeller University Press

Indexing

ISI Web of Knowledge - 68 Citations

Scopus - 65 Citations

Other information

Authenticus ID: P-006-85C

DOI: 10.1084/jem.20122842

Abstract (EN): DNA repair mechanisms are fundamental for B cell development, which relies on the somatic diversification of the immunoglobulin genes by V(D)J recombination, somatic hypermutation, and class switch recombination. Their failure is postulated to promote genomic instability and malignant transformation in B cells. By performing targeted sequencing of 73 key DNA repair genes in 29 B cell lymphoma samples, somatic and germline mutations were identified in various DNA repair pathways, mainly in diffuse large B cell lymphomas (DLBCLs). Mutations in mismatch repair genes (EXO1, MSH2, and MSH6) were associated with microsatellite instability, increased number of somatic insertions/deletions, and altered mutation signatures in tumors. Somatic mutations in nonhomologous end-joining (NHEJ) genes (DCLRE1C/ARTEMIS, PRKDC/DNA-PKcs, XRCC5/KU80, and XRCC6/KU70) were identified in four DLBCL tumors and cytogenetic analyses revealed that translocations involving the immunoglobulin-heavy chain locus occurred exclusively in NHEJ-mutated samples. The novel mutation targets, CHEK2 and PARP1, were further screened in expanded DLBCL cohorts, and somatic as well as novel and rare germline mutations were identified in 8 and 5% of analyzed tumors, respectively. By correlating defects in a subset of DNA damage response and repair genes with genomic instability events in tumors, we propose that these genes play a role in DLBCL lymphomagenesis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14