Capilia (TM) TB-Neo assay: a new tool for rapid distinction between tuberculous and non-tuberculous mycobacteria

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Ramos, A (Author) Other; Carvalho, T (Author) Other; Ribeiro, M (Author) Other; guimaraes, jt (Author) FMUP

Journal

Title: The International Journal of Tuberculosis and Lung Disease

Vol. 20

Pages: 753-756

ISSN: 1027-3719

Publisher: International Union Against Tuberculosis And Lung Disease

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00K-EYM

DOI: 10.5588/ijtld.15.0528

Abstract (EN): SETTING: The ability to rapidly distinguish between Mycobacterium tuberculosis complex (MTC) and non tuberculous mycobacteria (NTM) is critical in clinical practice. OBJECTIVE: To evaluate the usefulness of an immunochromatographic (IC) assay to distinguish between MTC and NTM. DESIGN: We analysed a panel of 145 cultures from 128 patients. The routine molecular identification approaches, such as the AccuProbe(TM) Mycobacterium tuberculosis complex culture identification test and GenoType(R) Mycobacterium assays, were used as reference methods. RESULTS: Of the 101 positive cultures, 98 were correctly identified using the Capilia(TM) TB-Neo Assay. Of the three discordant isolates, one was identified as M. bovis bacille Calmette-Guerin (BCG) and two as M. tuberculosis. Although we have not performed the sequencing of these strains, some false-negative results have been described due to mutations in the mpb64 gene or with some M. bovis BCG strains. We did not observe false-positive results or any cross-reaction with 22 NTM strains, 12 non-mycobacterial micro-organisms and 10 negative cultures. CONCLUSION: We report good overall performance (sensitivity 97%, specificity 100%, positive predictive value 100% and negative predictive value 96%) of this rapid assay that is easy to perform and interpret and does not require sample preparation, trained technicians or expensive equipment.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4