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Arabidopsis Thaliana Automatic Cell File Detection and Cell Length Estimation

Title
Arabidopsis Thaliana Automatic Cell File Detection and Cell Length Estimation
Type
Article in International Conference Proceedings Book
Year
2011
Authors
Pedro Quelhas
(Author)
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Jeroen Nieuwland
(Author)
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Walter Dewitte
(Author)
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Ana Maria Mendonca
(Author)
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Jim Murray
(Author)
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Aurelio Campilho
(Author)
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Conference proceedings International
Pages: 1-11
8th International Conference, ICIAR 2011
Burnaby, BC, Canada, June 22-24, 2011
Scientific classification
FOS: Engineering and technology > Electrical engineering, Electronic engineering, Information engineering
Other information
Authenticus ID: P-002-WYP
Abstract (EN): In plant development biology, the study of the structure of the plant's root is fundamental for the understanding of the regulation and interrelationships of cell division and cellular differentiation. This is based on the high connection between cell length and progression of cell differentiation and the nuclear state. However, the need to analyse a large amount of images from many replicate roots to obtain reliable measurements motivates the development of automatic tools for root structure analysis. We present a novel automatic approach to detect cell files, the main structure in plant roots, and extract the length of the cells in those files. This approach is based on the detection of local cell file characteristic symmetry using a wavelet based image symmetry measure. The resulting detection enables the automatic extraction of important data on the plant development stage and of characteristics for individual cells. Furthermore, the approach presented reduces in more than 90% the time required for the analysis of each root, improving the work of the biologist and allowing the increase of the amount of data to be analysed for each experimental condition. While our approach is fully automatic a user verification and editing stage is provided so that any existing errors may be corrected. Given five test images it was observed that user did not correct more than 20% of all automatically detected structure, while taking no more than 10% of manual analysis time to do so.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 11
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