O-glycan truncation enhances cancer-related functions of CD44 in gastric cancer

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Mereiter, S (Author) Other; Martins, AM (Author) Other; Gomes, C (Author) Other; Balmana, M (Author) Other; Macedo, JA (Author) Other; Polom, K (Author) Other; Roviello, F (Author) Other; Magalhaes, A (Author) Other; Celso Reis (Author) ICBAS

Journal

Title: FEBS Letters

Vol. 593

Pages: 1675-1689

ISSN: 0014-5793

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 18 Citations

Other information

Authenticus ID: P-00Q-X8J

DOI: 10.1002/1873-3468.13432

Abstract (EN): CD44 isoforms are often upregulated in gastric cancer and have been associated with increased metastatic potential and poor survival. To evaluate the functional impact of O-glycan truncation on CD44 we have analysed glyco-engineered cancer cell models displaying shortened O-glycans. Here, we demonstrate that induction of aberrant O-glycan termination through various molecular mechanisms affects CD44 molecular features. We show that CD44 is a major carrier of truncated O-glycans and that this truncation is accompanied by an increased hyaluronan binding capacity and affects extracellular shedding. In addition, short O-glycans promoted the colocalization of CD44v6 with the receptor tyrosine kinase RON and concomitantly increased activation. Our in vitro findings were validated in gastric cancer clinical samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15