Extracellular Vesicles Enriched in hsa-miR-301a-3p and hsa-miR-1293 Dynamics in Clear Cell Renal Cell Carcinoma Patients: Potential Biomarkers of Metastatic Disease

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Dias, F (Author) Other; Teixeira, AL (Author) Other; Nogueira, I (Author) Other; Morais, M (Author) Other; Maia, J (Author) Other; Bodo, C (Author) Other; Ferreira, M (Author) Other; Silva, A (Author) Other; Vilhena, M (Author) Other; Lobo, J (Author) Other; Sequeira, JP (Author) Other; Mauricio, J (Author) Other; Oliveira, J (Author) Other; Kok, K (Author) Other; Costa Silva, B (Author) Other; Rui Medeiros (Author) ICBAS

Journal

Title: Cancers

Vol. 12

Final page: 1450

ISSN: 2072-6694

Publisher: MDPI

Indexing

ISI Web of Knowledge - 35 Citations

Scopus - 34 Citations

Other information

Authenticus ID: P-00S-9J1

DOI: 10.3390/cancers12061450

Abstract (EN): Clear cell renal cell carcinoma (ccRCC) is the most aggressive subtype of kidney cancer and up to 40% of patients submitted to surgery with a curative intent will relapse. Thus, the aim of this study was to analyze the applicability of an Extracellular vesicle (EV) derived miRNA profile as potential prognosis biomarkers in ccRCC patients. We analyzed a nine-miRNA profile in plasma EVs from 32 ccRCC patients with localized disease (before and after surgery) and in 37 patients with metastatic disease. We observed that the levels of EV-derived hsa-miR-25-3p, hsa-miR-126-5p, hsa-miR-200c-3p, and hsa-miR-301a-3p decreased after surgery, whereas hsa-miR-1293 EV-levels increased. Furthermore, metastatic patients presented higher levels of hsa-miR-301a-3p and lower levels of hsa-miR-1293 when compared to patients with localized disease after surgery. Functional enrichment analysis of the targets of the four miRNAs that decreased after surgery resulted in an enrichment of terms related to cell cycle, proliferation, and metabolism, suggesting that EV-miRNA enrichment in the presence of the tumor could represent an epigenetic mechanism to sustain tumor development. Taken together, these results suggest that EVs content varies depending on the presence or absence of the disease and that an increase of EV-derived hsa-miR-301a-3p, and decrease of EV-derived hsa-miR-1293, may be potential biomarkers of metastatic ccRCC.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17