Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

• Type: Another Publication in an International Scientific Journal
• Date: 2022

Authors

Ribeiro, JC (Author) Other; Carrageta, DF (Author) Other; Oliveira, PF (Author) Other; Bernardino, RL (Author) ICBAS; Alves, MG (Author) Other

Journal

Title: Animals

Vol. 12

Final page: 359

Publisher: MDPI

Indexing

Scopus - 3 Citations

Other information

Authenticus ID: P-00W-1TH

DOI: 10.3390/ani12030359

Abstract (EN): Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function. The correct cryopreservation procedure is characterized by the balance between freezing rate and cryoprotective agents (CPAs), which minimizes cellular dehydration and intracellular ice formation. For this purpose, osmoregulation is a central process in cryopreservation. During cryopreservation, water and small solutes, including penetrating cryoprotective agents, cross the plasma membrane. Aquaporins (AQPs) constitute a family of channel proteins responsible for the transport of water, small solutes, and certain gases across biological membranes. Thirteen homologs of AQPs (AQP0-12) have been described. AQPs are widely distributed throughout the male and female reproductive systems, including the sperm and oocyte membrane. The composition of the male and female gamete membrane is of special interest for assisted reproductive techniques (ART), including cryopreservation. In this review, we detail the mechanisms involved in gamete cryopreservation, including the most used techniques and CPAs. In addition, the expression and function of AQPs in the male and female gametes are explored, highlighting the potential protective role of AQPs against damage induced during cryopreservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Language: English

Type (Professor's evaluation): Scientific