Mn-Based Methacrylated Gellan Gum Hydrogels for MRI-Guided Cell Delivery and Imaging

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Coimbra, S (Author) FCUP; Strymecka, P (Author) Other; Stanaszek, L (Author) Other; Silva-Correia, J (Author) Other; Drela, K (Author) Other; Fiedorowicz, M (Author) Other; Malysz-Cymborska, I (Author) Other; Janowski, M (Author) Other; Reis, RL (Author) Other; Lukomska, B (Author) Other; Walczak, P (Author) Other; Oliveira, JM (Author) Other

Journal

Title: Bioengineering

Vol. 10 No. 3

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus

Other information

Authenticus ID: P-00Y-BH4

DOI: 10.3390/bioengineering10040427

Abstract (EN): This work aims to engineer a new stable injectable Mn-based methacrylated gellan gum (Mn/GG-MA) hydrogel for real-time monitored cell delivery into the central nervous system. To enable the hydrogel visualization under Magnetic Resonance Imaging (MRI), GG-MA solutions were supplemented with paramagnetic Mn2+ ions before its ionic crosslink with artificial cerebrospinal fluid (aCSF). The resulting formulations were stable, detectable by T1-weighted MRI scans and also injectable. Cell-laden hydrogels were prepared using the Mn/GG-MA formulations, extruded into aCSF for crosslink, and after 7 days of culture, the encapsulated human adipose-derived stem cells remained viable, as assessed by Live/Dead assay. In vivo tests, using double mutant MBPshi/shi/rag2 immunocompromised mice, showed that the injection of Mn/GG-MA solutions resulted in a continuous and traceable hydrogel, visible on MRI scans. Summing up, the developed formulations are suitable for both non-invasive cell delivery techniques and image-guided neurointerventions, paving the way for new therapeutic procedures.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16