Expanded bed adsorption/desorption of proteins with streamline direct CST I adsorbent

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Li, P (Author) Other; Xiu, GH (Author) Other; Mata, VG (Author) Other; Grande, CA (Author) Other; Alírio Rodrigues (Author) FEUP

Journal

Title: Biotechnology and Bioengineering

Vol. 94 No. 6

Pages: 1155-1163

ISSN: 0006-3592

Publisher: Wiley-Blackwell

Indexing

ISI Proceedings

ISI Web of Knowledge - 19 Citations

Scopus - 16 Citations

Scientific classification

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

Authenticus ID: P-004-HWW

DOI: 10.1002/bit.20952

Resumo (PT): Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out for two-component proteins (BSA and myoglobin) competitive adsorption and desorption in an expanded bed packed with Streamline Direct CST I. Based on the measurements of the single- and two-component bovine serum albumin (BSA)/myoglobin adsorption isotherm on Streamline Direct CST I, the binding and elution conditions for the whole EBA process are selected; and then frontal analysis for a longer timescale and column displacement experiments in a fixed bed (XK16/20 column) are carried out to evaluate the two-component proteins (BSA and myoglobin) competitive adsorption and displacement on Streamline Direct CST I. Finally, the feasibility of capturing both BSA and myoglobin by an expanded bed packed with Streamline Direct CST I is addressed in a Streamline 50 column packed with 300 mL Streamline Direct CST I. (c) 2006 Wiley Periodicals, Inc.

Abstract (EN): Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out for two-component proteins (BSA and myoglobin) competitive adsorption and desorption in an expanded bed packed with Streamline Direct CST I. Based on the measurements of the single- and two-component bovine serum albumin (BSA)/myoglobin adsorption isotherm on Streamline Direct CST I, the binding and elution conditions for the whole EBA process are selected; and then frontal analysis for a longer timescale and column displacement experiments in a fixed bed (XK16/20 column) are carried out to evaluate the two-component proteins (BSA and myoglobin) competitive adsorption and displacement on Streamline Direct CST I. Finally, the feasibility of capturing both BSA and myoglobin by an expanded bed packed with Streamline Direct CST I is addressed in a Streamline 50 column packed with 300 mL Streamline Direct CST I. (c) 2006 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9