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Secretion capacity limitations of the Sec pathway in Escherichia coli

Title
Secretion capacity limitations of the Sec pathway in Escherichia coli
Type
Article in International Scientific Journal
Year
2004
Authors
Mergulhao, FJM
(Author)
Other
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Monteiro, GA
(Author)
Other
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Journal
Vol. 14 No. 1
Pages: 128-133
ISSN: 1017-7825
Publisher: Springer Nature
Indexing
Publicação em ISI Web of Science ISI Web of Science
Scientific classification
FOS: Natural sciences > Biological sciences
CORDIS: Technological sciences > Technology > Biotecnology
Other information
Authenticus ID: P-000-BTD
Abstract (EN): The secretion capacity of two E. coli strains (JM109 and AF1000) was evaluated through the expression of two human proinsulin fusion proteins using the translocation signal sequence from Staphylococcal protein A (SpA). Although a 7 to 11-fold difference in the expression levels was attained by the use of different promoters (SpA and malK promoters) and copy-number vectors (700 and 50 copies per cell), the maximum translocation rates for all the systems were around 140,000 amino acids cell(-1) min(-1). Moreover, the secretion capacity was found to be independent of the size of the exiting peptide and its translational rate.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 6
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