Determination of Ochratoxin A in Bread: Evaluation of Microwave-Assisted Extraction Using an Orthogonal Composite Design Coupled with Response Surface Methodology

• Type: Article in International Scientific Journal
• Date: 2013

Authors

P. Paíga (Author) Other; S. Morais (Author) Other; Maria Teresa Oliva-Teles (Author) Other; Manuela Correia (Author) Other; Cristina Delerue-Matos (Author) Other; A. M.M. Sousa (Author) FEUP; M. P. Gonçalves (Author) FEUP; Sofia C. Duarte (Author) Other; Angelina Pena (Author) Other; Celeste M. Lino (Author) Other

Journal

Title: Food and Bioprocess Technology

Vol. 6

Pages: 2466-2477

ISSN: 1935-5130

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 12 Citations

ISI Web of Science

Scopus - 11 Citations

Scientific classification

FOS: Engineering and technology > Other engineering and technologies

Other information

Authenticus ID: P-006-7QA

DOI: 10.1007/s11947-012-0876-8

Abstract (EN): An analytical method using microwave-assisted extraction (MAE) and liquid chromatography (LC) with fluorescence detection (FD) for the determination of ochratoxin A (OTA) in bread samples is described. A 2(4) orthogonal composite design coupled with response surface methodology was used to study the influence of MAE parameters (extraction time, temperature, solvent volume, and stirring speed) in order to maximize OTA recovery. The optimized MAE conditions were the following: 25 mL of acetonitrile, 10 min of extraction, at 80 A degrees C, and maximum stirring speed. Validation of the overall methodology was performed by spiking assays at five levels (0.1-3.00 ng/g). The quantification limit was 0.005 ng/g. The established method was then applied to 64 bread samples (wheat, maize, and wheat/maize bread) collected in Oporto region (Northern Portugal). OTA was detected in 84 % of the samples with a maximum value of 2.87 ng/g below the European maximum limit established for OTA in cereal products of 3 ng/g.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12