Lipid nanoparticles biocompatibility and cellular uptake in a 3D human lung model

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Magalhães, J (Author) Other; Pinheiro, M (Author) Other; Drasler, B (Author) Other; Septiadi, D (Author) Other; Petri Fink, A (Author) Other; Santos, SG (Author) Other; Rothen Rutishauser, B (Author) Other; Salette Reis (Author) FFUP

Journal

Title: Nanomedicine

Vol. 15 No. 6

Pages: 259-271

ISSN: 1743-5889

Publisher: Future Medicine Ltd.

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 3 Citations

Other information

Authenticus ID: P-00R-Q0A

DOI: 10.2217/nnm-2019-0256

Abstract (EN): Aim: Design nanostructured lipid carriers (NLC) to facilitate drug delivery to tuberculosis-infected areas, exploiting macrophage mannose receptors and assess their uptake in a 3D human lung model. Materials & methods: NLCs and mannosylated-NLCs were synthetized and characterized. Their uptake and biocompatibility were tested in a 3D human lung model. Results: The formulations have appropriate size (170-202 nm) and morphology for lung deposition. Cell membrane integrity was maintained and no significant pro-inflammatory cytokine (IL-1ß, IL-8 and TNF-¿) secretion or morphological changes were observed 24 h post nanoparticles exposure. NLCs and mannosylated NLCs were distributed in the apical side of the lung tissue, both in macrophages and in epithelial cells. Conclusion: NLCs are biocompatible carriers and can be used for pulmonary drug delivery. © 2019 Salette Rice.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13