Effect of 1-Carbaldehyde-3,4-dimethoxyxanthone on Prostate and HPV-18 Positive Cervical Cancer Cell Lines and on Human THP-1 Macrophages

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Rui Medeiros (Author) ICBAS; Horta, B (Author) Other; Freitas Silva, J (Author) Other; Silva, J (Author) Other; Dias, F (Author) Other; Emilia Sousa (Author) FFUP; Pinto, M (Author) Other; Cerqueira, F (Author) Other

Journal

Title: Molecules

Vol. 26 No. 17

Final page: 3721

ISSN: 1420-3049

Publisher: MDPI

Indexing

ISI Web of Knowledge - 2 Citations

Other information

Authenticus ID: P-00V-4CN

DOI: 10.3390/molecules26123721

Abstract (EN): Xanthone derivatives have shown promising antitumor properties, and 1-carbaldehyde-3,4-dimethoxyxanthone (1) has recently emerged as a potent tumor cell growth inhibitor. In this study, its effect was evaluated (MTT viability assay) against a new panel of cancer cells, namely cervical cancer (HeLa), androgen-sensitive (LNCaP) and androgen-independent (PC-3) prostate cancer, and nonsolid tumor derived cancer (Jurkat) cell lines. The effect of xanthone 1 on macrophage functions was also evaluated. The effect of xanthone 1-conditioned THP-1 human macrophage supernatants on the metabolic viability of cervical and prostate cancer cell lines was determined along with its interference with cytokine expression characteristic of M1 profile (IL-1 <= beta; TNF-alpha) or M2 profile (IL-10; TGF-beta) (PCR and ELISA). Nitric oxide (NO) production by murine RAW264.7 macrophages was quantified by Griess reaction. Xanthone 1 (20 mu M) strongly inhibited the metabolic activity of the cell lines and was significantly more active against prostate cell lines compared to HeLa (p < 0.05). Jurkat was the cell most sensitive to the effect of xanthone 1. Compound 1-conditioned IL-4-stimulated THP-1 macrophage supernatants significantly (p < 0.05) inhibited the metabolic activity of HeLa, LNCaP, and PC-3. Xanthone 1 did not significantly affect the expression of cytokines by THP-1 macrophages. The inhibiting effect of compound 1 observed on the production of NO by RAW 264.7 macrophages was moderate. In conclusion, 1-carbaldehyde-3,4-dimethoxyxanthone (1) decreases the metabolic activity of cancer cells and seems to be able to modulate macrophage functions.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11