Metabolic profiling of induced acute pancreatitis and pancreatic cancer progression in a mutant Kras mouse model

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Carneiro, TJ (Author) Other; Pinto, J (Author) Other; Serrao, EM (Author) Other; Barros, AS (Author) FMUP; Brindle, KM (Author) Other; Gil, AM (Author) Other

Journal

Title: Frontiers in Molecular Biosciences

Vol. 9

Publisher: Frontiers Media

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00X-2QX

DOI: 10.3389/fmolb.2022.937865

Abstract (EN): Untargeted Nuclear Magnetic Resonance (NMR) metabolomics of polar extracts from the pancreata of a caerulin-induced mouse model of pancreatitis (Pt) and of a transgenic mouse model of pancreatic cancer (PCa) were used to find metabolic markers of Pt and to characterize the metabolic changes accompanying PCa progression. Using multivariate analysis a 10-metabolite metabolic signature specific to Pt tissue was found to distinguish the benign condition from both normal tissue and precancerous tissue (low grade pancreatic intraepithelial neoplasia, PanIN, lesions). The mice pancreata showed significant changes in the progression from normal tissue, through low-grade and high-grade PanIN lesions to pancreatic ductal adenocarcinoma (PDA). These included increased lactate production, amino acid changes consistent with enhanced anaplerosis, decreased concentrations of intermediates in membrane biosynthesis (phosphocholine and phosphoethanolamine) and decreased glycosylated uridine phosphates, reflecting activation of the hexosamine biosynthesis pathway and protein glycosylation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16