Cytotoxic effect of the serotonergic drug 1-(1-Naphthyl)piperazine against melanoma cells

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Menezes, AC (Author) Other; Carvalheiro, M (Author) Other; de Oliveira, JMPF (Author) FCUP; Ascenso, A (Author) Other; Oliveira, H (Author) Other

Journal

Title: Toxicology in Vitro

Vol. 47

Pages: 72-78

ISSN: 0887-2333

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00N-8N1

DOI: 10.1016/j.tiv.2017.11.011

Abstract (EN): 1-(1-Naphthyl)piperazine (1-NPZ) is a serotonergic derivative of quipazine acting both as antagonist and agonist of different serotonin receptors, with promising results for the management of skin cancer. In this work, we studied the effect of 1-NPZ on human MNT-1 melanoma cells by evaluating its effects on cell viability, ability to form colonies, cell cycle dynamics, reactive oxygen species (ROS) production and apoptosis. Treatment of MNT-1 cells with 1-NPZ for 24 h decreased cell viability and induced apoptosis in a dose-dependent manner. Activity against melanoma was confirmed with a different melanoma cell line, SK-MEL-28. Simultaneously, 1-NPZ affected cell cycle progression by mediating a S-phase delay. Higher levels of ROS were also detected in MNT-1 cells after treatment with 1-NPZ. Furthermore, 1-NPZ significantly increased the expression of cyclooxygenase-2 in MNT-1 cells. These findings suggest that 1-NPZ pretreatment is able to induce oxidative stress, and consequently apoptotic cell death in melanoma cells. In conclusion, this study demonstrates the cytotoxic and genotoxic potential of 1-NPZ against melanoma cells.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7