OPTIMISATION OF REACTIVE DYE DEGRADATION BY LACCASE USING BOX-BEHNKEN DESIGN

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Raquel O. Cristóvão (Author) Other; Ana P. M. Tavares (Author) FEUP; José Miguel Loureiro (Author) FEUP; Rui Boaventura (Author) FEUP; Maria Eugénia Macedo (Author) FEUP

Journal

Title: Environmental Technology (United Kingdom)

Vol. 29

Pages: 1357-1364

ISSN: 0959-3330

Publisher: Taylor & Francis

Indexing

ISI Proceedings

ISI Web of Knowledge - 18 Citations

ISI Web of Science

Scopus - 23 Citations

COMPENDEX

INSPEC

Scientific classification

FOS: Engineering and technology > Chemical engineering

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

Authenticus ID: P-004-358

DOI: 10.1080/09593330802379615

Abstract (EN): A three-level Box-Behnken factorial design with three factors and the Response Surface Methodology were used to optimise the colour removal of the reactive textile dye, Colour Index (C.I.) reactive red 180, by commercial laccase. A mathematical model was developed to study the effect of temperature, pH, enzyme concentration and their interactions on the decolourisation. Enzyme concentration and pH as well as their interaction were the principal factors that affected the decolourisation. The dye degradation was independent of temperature. The model estimated that the highest decolourisation ( 92%) was obtained for 27C, pH 7.5 and 85 U l-1. This predicted value was experimentally validated, obtaining dye colour removal (540 nm) of 93 1.5%.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8