Kitul, a food plant with antidiabetic-like effects: Reduction of intracellular reactive species in glucose-stimulated RIN-5F pancreatic beta-cells and mitigation of pro-inflammatory mediators in activated RAW 264.7 macrophages

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Catarina Andrade (Author) Other; David M Pereira (Author) FFUP; Nelson G. M. Gomes (Author) FFUP; Federico Ferreres (Author) Other; Angel Gil-Izquierdo (Author) Other; Paula B Andrade (Author) FFUP; Sutsawat Duangsrisai (Author) Other; Patricia Valentao (Author) FFUP

Journal

Title: Food Research International

Vol. 167

ISSN: 0963-9969

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus

Other information

Authenticus ID: P-00Y-2C9

DOI: 10.1016/j.foodres.2023.112615

Abstract (EN): Kitul (Caryota urens L.) inflorescences are broadly used for sweet sap production in Asian countries and Kitul food products are known as being suitable for diabetic patients. Considering the strong ability to inhibit alpha-glucosidase, we hypothesize that kitul antidiabetic properties might also involve the modulation of inflammatory pathways and hyperglycaemia-induced oxidative damage. Hence, the effects of an inflorescence's methanol extract were investigated in glucose-stimulated pancreatic cells (RIN-5F) and LPS-stimulated RAW 264.7 macrophages. The extract reduced the overproduction of intracellular reactive species in pancreatic cells and also NO, L-citrulline and IL-6 levels in LPS-stimulated RAW 264.7 macrophages. Inhibition of 5-lipoxygenase (IC50 = 166.1 mu g/mL) through an uncompetitive manner was also recorded upon treatment with C. urens inflorescences extract. The phenolic profile of the inflorescences was characterized by HPLC-DAD, six hydroxycinnamic acids being iden-tified and quantified. Overall, our data provide additional evidence on the pleiotropic mechanisms of Kitul in-florescences as an antidiabetic agent.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10