Nanoparticle-Delivered 2-PAM for Rat Brain Protection against Paraoxon Central Toxicity

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Pashirova, TN (Author) Other; Zueva, IV (Author) Other; Petrov, KA (Author) Other; Babaev, VM (Author) Other; Lukashenko, SS (Author) Other; Rizvanov, IK (Author) Other; Souto, EB (Author) Other; Nikolsky, EE (Author) Other; Zakharova, LY (Author) Other; Masson, P (Author) Other; Sinyashin, OG (Author) Other

Journal

Title: ACS Applied Materials & Interfaces

Vol. 9 No. 28

ISSN: 1944-8244

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 44 Citations

Scopus - 26 Citations

Other information

Authenticus ID: P-00M-VXQ

DOI: 10.1021/acsami.7b04163

Abstract (EN): Solid lipid nanoparticles (SLNs) are among the most promising nanocarriers to target the blood-brain barrier (BBB) for drug delivery to the central nervous system (CNS). Encapsulation of the acetylcholinesterase reactivator, pralidoxime chloride (2-PAM), in SLNs appears to be a suitable strategy for protection against poisoning by organophosphorus agents (OPs) and postexposure treatment. 2-PAM-loaded SLNs were developed for brain targeting and delivery via intravenous (iv) administration. 2-PAM-SLNs displayed a high 2-PAM encapsulation efficiency (~90%) and loading capacity (maximum 30.8 ± 1%). Drug-loaded particles had a mean hydrodynamic diameter close to 100 nm and high negative zeta potential (-54 to -15 mV). These properties contribute to improve long-term stability of 2-PAM-SLNs when stored both at room temperature (22 °C) and at 4 °C, as well as to longer circulation time in the bloodstream compared to free 2-PAM. Paraoxon-poisoned rats (2 × LD50) were treated with 2-PAM-loaded SLNs at a dose of 2-PAM of 5 mg/kg. 2-PAM-SLNs reactivated 15% of brain AChE activity. Our results confirm the potential use of SLNs loaded with positively charged oximes as a medical countermeasure both for protection against OPs poisoning and for postexposure treatment. © 2017 American Chemical Society.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12