Solid lipid nanoparticles optimized by 2(2) factorial design for skin administration: Cytotoxicity in NIH3T3 fibroblasts

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Rigon, RB (Author) Other; Goncalez, ML (Author) Other; Severino, P (Author) Other; Alves, DA (Author) Other; Santana, MHA (Author) Other; Souto, EB (Author) Other; Chorilli, M (Author) Other

Journal

Title: Colloids and Surfaces B: Biointerfaces

Vol. 171

Pages: 501-505

ISSN: 0927-7765

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 33 Citations

Other information

Authenticus ID: P-00P-E0E

DOI: 10.1016/j.colsurfb.2018.07.065

Abstract (EN): The present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 2(2) full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulation. The effect of the composition of lipid and water phases on the mean particle size (z-AVE), polydispersity index (PdI) and zeta potential (ZP) was studied. The developed formulations were composed of 5.0% of lipid phase (stearic acid (SA), behenic alcohol (BA) or a blend of SA:BA (1:1)) and 4.7% of surfactants (soybean phosphatidylcholine and poloxamer 407). In vitro cytotoxicity using NIH3T3 fibroblasts was performed by MTT reduction assay. This factorial design study has proven to be a useful tool in optimizing SLN (z-AVE similar to 200 nm), which were shown to be non-cytotoxic. The present results highlight the benefit of applying statistical designs in the preparation and optimization of SLN formulations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5