Release kinetics and cell viability of ibuprofen nanocrystals produced by melt-emulsification

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Fernandes, AR (Author) Other; Dias Ferreira, J (Author) Other; Cabral, C (Author) Other; Garcia, ML (Author) Other; Souto, EB (Author) Other

Journal

Title: Colloids and Surfaces B: Biointerfaces

Vol. 166

Pages: 24-28

ISSN: 0927-7765

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 17 Citations

Other information

Authenticus ID: P-00N-Q9F

DOI: 10.1016/j.colsurfb.2018.03.005

Abstract (EN): The clinical use of poorly water-soluble drugs has become a big challenge in pharmaceutical development due to the compromised bioavailability of the drugs in vivo. Nanocrystals have been proposed as a formulation strategy to improve the dissolution properties of these drugs. The benefits of using nanocrystals in drug delivery, when compared to other nanoparticles, are related to their production facilities, simple structure, and suitability for a variety of administration routes. High pressure homogenization (HPH) is the most promising production process, which can be employed at low or high temperatures. Ibuprofen nanocrystals with a mean size below 175 nm, and polydispersity below 0.18, have been produced by melt emulsification, followed by HPH. Two nanocrystal formulations, differing on the surfactant composition, have been produced, their in vitro ibuprofen release tested in Franz diffusion cells and adjusted to several kinetic models (zero order, first order, Higuchi, Hixson-Crowell, Korsmeyer-Peppas, Baker-Lonsdale and Weibull model). Cell viability was assessed at 3, 6 and 24h of incubation on human epithelial colorectal cells (Caco-2) by AlamarBlue colorimetric assay. For both formulations, Caco-2 cells viability was dependent on the drug concentration and time of exposure.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5