Africanized Bee Venom (Apis mellifera Linnaeus): Neuroprotective Effects in a Parkinson's Disease Mouse Model Induced by 6-hydroxydopamine

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Dantas, CG (Author) Other; da Paixao, AO (Author) Other; Nunes, TLGM (Author) Other; Silva, IJF (Author) Other; Lima, BD (Author) Other; Araujo, AAS (Author) Other; de Albuquerque, RLC (Author) Other; Gramacho, KP (Author) Other; Padilha, FF (Author) Other; da Costa, LP (Author) Other; Severino, P (Author) Other; Cardoso, JC (Author) Other; Souto, EB (Author) FFUP; Gomes, MZ (Author) Other

Journal

Title: Toxics

Vol. 15

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 0 Citations

Other information

Authenticus ID: P-00X-DDQ

DOI: 10.3390/toxics10100583

Abstract (EN): This study evaluated the neuroprotective effects of the Africanized bee venom (BV) and its mechanisms of action after 6-hydroxydopamine-(6-OHDA)-induced lesion in a mice model. Prior to BV treatment, mice received intrastriatal microinjections of 6-OHDA (no induced dopaminergic neuronal death) or ascorbate saline (as a control). BV was administered subcutaneously at different dosages (0.01, 0.05 or 0.1 mg.Kg(-1)) once every two days over a period of 3 weeks. The open field test was carried out, together with the immunohistochemical and histopathological analysis. The chemical composition of BV was also assessed, identifying the highest concentrations of apamin, phospholipase A(2) and melittin. In the behavioral evaluation, the BV (0.1 mg.Kg(-1)) counteracted the 6-OHDA-induced decrease in crossings and rearing. 6-OHDA caused loss of dopaminergic cell bodies in the substantia nigra pars compacta and fibers in striatum (STR). Mice that received 0.01 mg.Kg(-1) showed significant increase in the mean survival of dopaminergic cell bodies. Increased astrocytic infiltration occurred in the STR of 6-OHDA injected mice, differently from those of the groups treated with BV. The results suggested that Africanized BV has neuroprotective activity in an animal model of Parkinson's disease.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18