Chemometric-assisted surface-enhanced Raman spectroscopy for metformin determination using gold nanoparticles as substrate

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Castro, RC (Author) Other; Ribeiro, DSM (Author) Other; Joao L M Santos (Author) FFUP; Nunes, C (Author) Other; Salette Reis (Author) FFUP; Pascoa, RNMJ (Author) Other

Journal

Title: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

Vol. 287

ISSN: 1386-1425

Publisher: Elsevier

Indexing

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Other information

Authenticus ID: P-00X-G30

DOI: 10.1016/j.saa.2022.122118

Abstract (EN): A fast, simple, and reliable method for determination of metformin was developed by coupling surface-enhanced Raman spectroscopy (SERS) with chemometric methods. This relayed on the utilization of a portable Raman spectrometer and of citrate stabilized gold nanoparticles (AuNPs) as substrate, to carry out the measurement of SERS scattering signals, thus assuring improved sensitivity. The obtained datasets were analysed using principal component analysis (PCA) and partial least squares (PLS) regression. Upon optimization of the PLS model, in terms of latent variables, spectral region and pre-processing techniques, RMSECV and R2CV values of 0.42 mg/L and 0.94, respectively, were obtained. The optimized PLS regression model was further validated with the projection of commercial pharmaceutical samples, providing good results in terms of R2P (0.97), RE (4.54 %) and analytical sensitivity (2.13 mg/L).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7