Differential Refractometric Biosensor for Reliable Human IgG Detection: Proof of Concept

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Mendes, JP (Author) Other; Coelho, LCC (Author) FCUP; Jorge, PAS (Author) FCUP; Carlos M Pereira (Author) FCUP

Journal

Title: Biosensors-Basel

Vol. 12

Final page: 515

ISSN: 2079-6374

Publisher: MDPI

Indexing

ISI Web of Knowledge - 9 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00W-WKS

DOI: 10.3390/bios12070515

Abstract (EN): A new sensing platform based on long-period fiber gratings (LPFGs) for direct, fast, and selective detection of human immunoglobulin G (IgG; Mw = 150 KDa) was developed and characterized. The transducer's high selectivity is based on the specific interaction of a molecularly imprinted polymer (MIPs) design for IgG detection. The sensing scheme is based on differential refractometric measurements, including a correction system based on a non-imprinted polymer (NIP)-coated LPFG, allowing reliable and more sensitive measurements, improving the rejection of false positives in around 30%. The molecular imprinted binding sites were performed on the surface of a LPFG with a sensitivity of about 130 nm/RIU and a FOM of 16 RIU-1. The low-cost and easy to build device was tested in a working range from 1 to 100 nmol/L, revealing a limit of detection (LOD) and a sensitivity of 0.25 nmol/L (0.037 mu g/mL) and 0.057 nm.L/nmol, respectively. The sensor also successfully differentiates the target analyte from the other abundant elements that are present in the human blood plasma.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 18