Optical Biosensor for the Detection of Biogenic Amines

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Vasconcelos, HCASG (Author) Other; de Almeida, JMMM (Author) FCUP; Mendes, JP (Author) Other; Dias, B (Author) Other; Jorge, PAS (Author) FCUP; Saraiva, CMT (Author) Other; Coelho, LCC (Author) FCUP

Journal

Title: IEEE Sensors Journal

Vol. 22

ISSN: 1530-437X

Publisher: IEEE

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 5 Citations

Other information

Authenticus ID: P-00X-4T7

DOI: 10.1109/jsen.2022.3204900

Abstract (EN): Biogenic amines (BAs) are compounds found in a vast range of food products. In recent years, there has been a crescent awareness toward food safety, followed by an increase in food regulations. Long-period fiber gratings (LPFGs) coated with titanium dioxide (TiO2) were used to monitor the optical properties of a layer of poly(ethylene-co-vinyl acetate) (PEVA) doped with maleic anhydride (MA), which was polymerized on top of TiO2. This hydrophobic polymeric structure is permeable to BA, which causes a steady increase in its effective refractive index (RI) causing a wavelength shift in the coated LPFG attenuation band. LPFG wavelength shift was observed and measured for the monoamine tyramine (TYR), to the diamines, putrescine (PUT), cadaverine (CAD), histamine (HIS), and tryptamine (TRYP), and to the polyamines, spermidine (SPED), and spermine (SPEM). It was determined that, while PEVA-coated devices present a residual sensitivity to BA, the MA greatly increases it. In fact, for PEVA only coated LPFGs, the sensitivities of 1.45 +/- 0.11, 0.97 +/- 0.05, 0.46 +/- 0.08, and 0.94 +/- 0.09 nmM-1 for PUT, CAD, HIS, and TYR, respectively, were measured. However, for PEVA-doped MA-coated LPFGs, the sensitivities are 3.34 +/- 0.13, 3.06 +/- 0.11, 2.62 +/- 0.14, and 3.65 +/- 0.23 nmM-1 for PUT, CAD, HIS, and TYR, respectively. Thus, the RI of PEVA increases with BAs in- diffusion, and MA doping further enhances the PEVA sensitivity to BA. The proposed sensor is expected to play a part in the further development of a biosensor for the quantification of BA in real foodstuff, providing a methodology for quality control.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8