A combined experimental and computational study to discover novel tyrosinase inhibitors

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Amaral, LMPF (Author) FCUP; Moniz, T (Author) ICBAS; Leite, A (Author) Other; Oliveira, A (Author) Other; Pedro A Fernandes (Author) FCUP; Ramos, MJ (Author) FCUP; Araujo, AN (Author) FFUP; Freitas, M (Author) Other; Eduarda Fernandes (Author) FFUP; Maria Rangel (Author) ICBAS

Journal

Title: Journal of Inorganic Biochemistry

Vol. 234

ISSN: 0162-0134

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00W-PVQ

DOI: 10.1016/j.jinorgbio.2022.111879

Abstract (EN): Depigmenting properties of tyrosinase inhibitors (TAi) boosted the search for new compounds applicable in cosmetics. Kojic acid, a 3-hydroxy-4-pyrone, is the most studied tyrosinase inhibitor but undesirable side effects, like dermatitis, and unspecified mechanism led to its exclusion in several countries. To discover safer and more efficient TA, we evaluated tyrosinase inhibitory effect of twelve 3-hydroxy-4-pyridinones (3,4-HPO) in vitro and considering the two reaction steps of inhibition in mushroom tyrosinase enzyme. In parallel we performed molecular docking studies in human and mushroom enzymes. Ligands I6 and I11 were the most effective compounds considering their inhibitory activity in both reaction steps. Our studies revealed that I6 has a non-competitive and mixed type of inhibition for monophenolase and diphenolase activity, while ligand I11 showed a mixed and competitive inhibition type for each reaction step. Molecular Docking results indicated that ligands tend to bind the enzyme by coordinating directly with the binuclear cooper centre and highlighted the relevance of voluminous and non-polar substituents at R-2 to avoid the binding of the ligands to the enzyme. The work clarifies the type of inhibition established for kojic acid and points out the differences found for the set of 3,4-HPO chelators studied as prospective tyrosinase inhibitors.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11