Adhesion and growth of human schwann cells on trimethylene carbonate (co)polymers

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Pego, AP (Author) Other; Vleggeert Lankamp, CLAM (Author) Other; Deenen, M (Author) Other; Lakke, EAJF (Author) Other; Grijpma, DW (Author) Other; Poot, AA (Author) Other; Marani, E (Author) Other; Feijen, J (Author) Other

Journal

Title: Journal of Biomedical Materials Research

Vol. 67A

Pages: 876-885

ISSN: 0021-9304

Publisher: Heterocorporation

Indexing

ISI Web of Knowledge - 39 Citations

ISI Web of Science

Scopus - 41 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

Other information

Authenticus ID: P-008-NGY

DOI: 10.1002/jbm.a.10074

Abstract (EN): Seeding of artificial nerve grafts with Schwann cells is a promising strategy for bridging large nerve defects. The aim of the present study was to evaluate the adhesion and growth of human Schwann cells (HSCs) on 1,3-trimethylene carbonate (TMC) and epsilon-caprolactone copolymers, with the final goal of using these materials in the development of an artificial nerve graft. The adhesion, proliferation, and morphology of HSCs on copolymers containing 10 and 82 mol % of TMC and on the parent homopolymers were investigated. HSCs adhered faster and in greater numbers on the copolymer with 82 mol % of TMC and on the TMC homopolymer compared with the other (co)polymers. On all polymer films, cell adhesion was lower than on gelatin (positive control). Despite differences in cell adhesion, cells displayed exponential growth on all tested surfaces, with similar growth rates. Cell numbers doubled approximately every 3 days on all substrates. When the polymer films were coated with fibronectin, no significant differences in cell adhesion and proliferation were observed between coated polymer surfaces and gelatin. The results indicate that all tested materials support the adhesion and proliferation of HSCs and can in principle be used for the preparation of flexible and slowly degrading nerve guides. (C) 2003 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10