The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Chorny, A (Author) Other; Casas Recasens, S (Author) Other; Sintes, J (Author) Other; Shan, M (Author) Other; Polentarutti, N (Author) Other; Garcia Escudero, R (Author) Other; Walland, AC (Author) Other; Yeiser, JR (Author) Other; Cassis, L (Author) Other; Carrillo, J (Author) Other; Puga, I (Author) Other; Cunha, C (Author) Other; Bastos, H (Author) Other; Rodrigues, F (Author) Other; Lacerda, JF (Author) Other; Morais, A (Author) Other; Dieguez Gonzalez, R (Author) Other; Heeger, PS (Author) Other; Salvatori, G (Author) Other; Carvalho, A (Author) Other; Garcia Sastre, A (Author) Other; Blander, JM (Author) Other; Mantovani, A (Author) Other; Garlanda, C (Author) Other; Cerutti, A (Author) Other

Journal

Title: Journal of Experimental Medicine

Vol. 213

Pages: 2167-2185

ISSN: 0022-1007

Publisher: Rockefeller University Press

Indexing

ISI Web of Knowledge - 43 Citations

Scopus - 45 Citations

Other information

Authenticus ID: P-00M-3CV

DOI: 10.1084/jem.20150282

Abstract (EN): Pentraxin 3 (PTX3) is a fluid-phase pattern recognition receptor of the humoral innate immune system with ancestral antibody-like properties but unknown antibody-inducing function. In this study, we found binding of PTX3 to splenic marginal zone (MZ) B cells, an innate-like subset of antibody-producing lymphocytes strategically positioned at the interface between the circulation and the adaptive immune system. PTX3 was released by a subset of neutrophils that surrounded the splenic MZ and expressed an immune activation-related gene signature distinct from that of circulating neutrophils. Binding of PTX3 promoted homeostatic production of IgM and class-switched IgG antibodies to microbial capsular polysaccharides, which decreased in PTX3-deficient mice and humans. In addition, PTX3 increased IgM and IgG production after infection with blood-borne encapsulated bacteria or immunization with bacterial carbohydrates. This immunogenic effect stemmed from the activation of MZ B cells through a neutrophil-regulated pathway that elicited class switching and plasmablast expansion via a combination of T cell-independent and T cell-dependent signals. Thus, PTX3 may bridge the humoral arms of the innate and adaptive immune systems by serving as an endogenous adjuvant for MZ B cells. This property could be harnessed to develop more effective vaccines against encapsulated pathogens.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 19