Effects of Co-Cr corrosion products and corresponding separate metal ions on human osteoblast-like cell cultures

• Type: Article in International Scientific Journal
• Date: 1996

Authors

Tomas, H (Author) Other; Carvalho, GS (Author) Other; Fernandes MH (Author) FMDUP; Freire, AP (Author) Other; Abrantes, LM (Author) Other

Journal

Title: Journal of Materials Science: Materials in Medicine

Vol. 7 No. 3

Pages: 291-296

ISSN: 0957-4530

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 23 Citations

Other information

Authenticus ID: P-001-ECE

DOI: 10.1007/bf00058569

Abstract (EN): The cytocompatibility of the degradation products of a Co-Cr orthopaedic alloy was investigated with particular focus on the dose-effect of an electrochemically dissolved alloy extract and of the corresponding separate metal ions on human osteogenic bone marrow derived cells. The extract solution contained 15 ppm of Co and 8 ppm of Cr as analysed by atomic absorption spectroscopy. Stock salt solutions of CoCl2 . 6H(2)O, CrCl3 . 6H(2)O and Na2CrO4 at corresponding concentrations were also prepared. Several dilutions of the above metallic solutions were tested for a period of 21 days on cells (third subculture) cultured in alpha-minimal essential medium containing foetal bovine serum and supplemented with antibiotics, dexamethasone ascorbic acid and beta-glycerophosphate. The osteoblast response to the presence of metal ions was evaluated by several biochemical parameters: cell viability (MTT reduction by intracellular enzymes), alkaline phosphatase activity (an osteoblast marker) and protein production (both intracellular and extracellular). Co-Cr corrosion products showed opposite effects to their respective metal salts only on day 1. With time the different metal solutions presented a similar pattern of inhibition. These results suggest that impaired bone formation in vitro can occur in the presence of Co-Cr corrosion products.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6