In vitro evaluation of permeation, toxicity and effect of praziquantel-loaded solid lipid nanoparticles against Schistosoma mansoni as a strategy to improve efficacy of the schistosomiasis treatment

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Ribeiro de Souza, ALR (Author) Other; Andreani, T (Author) Other; de Oliveira, RN (Author) Other; Kiill, CP (Author) Other; dos Santos, FK (Author) Other; Allegretti, SM (Author) Other; Chaud, MV (Author) Other; Souto, EB (Author) Other; Silva, AM (Author) Other; Daflon Gremiao, MPD (Author) Other

Journal

Title: International Journal of Pharmaceutics

Vol. 463

Pages: 31-37

ISSN: 0378-5173

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 60 Citations

Scopus - 64 Citations

Other information

Authenticus ID: P-009-3EN

DOI: 10.1016/j.ijpharm.2013.12.022

Abstract (EN): Solid lipid nanoparticles (SLN) are a promising drug delivery system for oral administration of poorlywater soluble drugs because of their capacity to increase the solubility of drug molecules when loaded in their lipid matrices, with the resulting improvement of the drug bioavailability. In the present work, we have developed praziquantel (PZQ)-loaded SLN and explored the biological applications of this system for intestinal permeation of PZQQ. The effect in vitro on Schistosoma mansoni culture and the cytotoxicity in HepG2 line cell were also evaluated. The results showed a significant decrease in the intestinal absorption of PZQloaded in SLN compared to free PZQ, suggesting that the SLN matrix could act as reservoir system. In culture of S. mansoni, we observed that PZQ-loaded SLN were more effective than free PZQ, leading the death of the parasites in less time. The result was proportional to doses of PZQ (25 and 50 tig mL-1) and lipid concentration. Regarding cytotoxicity, the encapsulation of PZQinto SLN decreased the toxicity in HepG2 cells in comparison to the free PZQ. From the obtained results, PZQ-loaded SLN could be a new drug delivery system for the schistosomiasis treatment especially in marginalized communities, improving the therapeutic efficacy and reducing the toxic effects of PZQ. (C) 2014 Published by Elsevier B.V.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7