Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Severino, P (Author) Other; Silva, H (Author) Other; Souto, EB (Author) Other; Santana, MHA (Author) Other; Dalla Costa, TCT (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: Journal of Pharmaceutical Analysis

Vol. 2

Pages: 29-34

ISSN: 2095-1779

Indexing

Scopus - 3 Citations

Other information

Authenticus ID: P-00G-2B8

DOI: 10.1016/j.jpha.2011.10.006

Abstract (EN): Didanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV). An automated system using on-line solid extraction and High Performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosine in dog plasma. Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma. Extraction was carried out on C18 cartridges, with high extraction yield as stationary phase, whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer, acetonitrile (KH2PO4: acetonitrile: 96:4, v/v) and 0.5% (w/v) of heptane sulphonic acid. The pH was adjusted to 6.5 with triethylamine. All samples and standard solutions were chromatographed at 28 °C. For an isocratic run, the flux was 1.0 mL/min, detection was at 250 nm and injected volume was 20 ¿L. The method was selective and linear for concentrations between 50 and 5000 ng/mL. Drug stability data ranged from 96% to 98%, and limit of quantification was 25 ng/mL. Extraction yield was up to 95%. Drug stability in dog plasma was kept frozen at -20 °C for one month after three freezethaw cycles, and for 24 h after processing in the auto sampler. Assay was successfully applied to measure didanosine concentrations in plasma dogs.

Language: English

Type (Professor's evaluation): Scientific