Sodium alginate-cross-linked polymyxin B sulphate-loaded solid lipid nanoparticles: Antibiotic resistance tests and HaCat and NIH/3T3 cell viability studies

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Severino, P (Author) Other; Chaud, MV (Author) Other; Shimojo, A (Author) Other; Antonini, D (Author) Other; Lancelloti, M (Author) Other; Santana, MHA (Author) Other; Souto, EB (Author) Other

Journal

Title: Colloids and Surfaces B: Biointerfaces

Vol. 129

Pages: 191-197

ISSN: 0927-7765

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 28 Citations

Scopus - 51 Citations

Other information

Authenticus ID: P-00G-5C8

DOI: 10.1016/j.colsurfb.2015.03.049

Abstract (EN): Polymyxins are a group of antibiotics with a common structure of a cyclic peptide with a long hydrophobic tail. Polymyxin B sulphate (PLX) has cationic charge, which is an obstacle for the efficient loading into Solid Lipid Nanoparticles (SLN). In the present paper, we describe an innovative method to load PLX into SLN to achieve the sustained release of the drug. PLX was firstly cross-linked with sodium alginate (SA) at different ratios (1:1, 1:2 and 1:3 SA/PLX), and loaded into SLN produced by high pressure homogenization (HPH). Optimized SLN were produced applying 500 bar pressure and 5 homogenization cycles. The best results were obtained with SA/PLX (1:1), recording 99.08 +/- 1.2% for the association efficiency of the drug with SA, 0.99 +/- 10 g for the loading capacity and 212.07 +/- 5.84% degree of swelling. The theological profile of aqueous SA solution followed the typical behaviour of concentrated polymeric solutions, whereas aqueous SA/PLX solution exhibited a gel-like dynamic behaviour. Micrographs show that SA/PLX depicted a porous and discontinuous amorphous phase in different ratios. The encapsulation efficiency of SA/PLX (1:1) in SLN, the mean particle diameter, polydispersity index and zeta potential were, respectively, 82.7 +/- 5.5%; 439.5 +/- 20.42 nm, 0.241 +/- 0.050 and -34.8 +/- 0.55 my. The effect of SLN on cell viability was checked in HaCat and NIH/3T3 cell lines, and the minimal inhibitory concentrations (MIC) were determined in Pseudomonas aeruginosa strains. SA/PLX-loaded SLN were shown to be less toxic than free PLX. Minimal inhibitory concentrations (MIC) showed the presence of the cross-linker polymer-drug complex, and SLN were shown to enhance MIC in the evaluated strains.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7