Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Doktorovova, S (Author) Other; Silva, AM (Author) Other; Gaivao, I (Author) Other; Souto, EB (Author) Other; Teixeira, JP (Author) Other; Martins Lopes, P (Author) Other

Journal

Title: Journal of Applied Toxicology

Vol. 34 No. 6

Pages: 395-403

ISSN: 0260-437X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 36 Citations

Other information

Authenticus ID: P-009-4AA

DOI: 10.1002/jat.2961

Abstract (EN): Cationic solid lipid nanoparticles (cSLN) are colloidal carriers for genes or drugs, particularly lipophilic drugs. Several reports exist on their high efficiency, but only a few studies report the effect of cSLNs on living cells. In the present work, internalization, cell viability (alamar blue assay) and genotoxic potential (alkaline comet assay) of three cSLN formulations (A-C) were evaluated in HepG2 and Caco-2 cells. cSLN showed an average hydrodynamic diameter (z-ave) of 141-222nm, zeta-potential of 55.0-72.5mV and polidispersity indices (PdI) of 0.336-0.421. Dispersion in physiological buffers increased z-ave and PdI. 0.01mgml(-1) cSLN unaffected cell viability, but 1.0mgml(-1) significantly decreased it, being cSLN-C (Compritol-based) the most toxic and HepG2 the most affected. DNA damage was not significantly increased by 0.1mgml(-1) cSLN but damage was observed at 1.0mgml(-1) cSLN-C. Thus, no genotoxicity is to be expected at concentrations that do not reduce cell viability. Copyright (c) 2013 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9