Modelling Hyperglycaemia in an Epithelial Membrane Model: Biophysical Characterisation

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Reis, A (Author) Other; Teixeira, JPF (Author) Other; Silva, AMG (Author) FCUP; Ferreira, M (Author) Other; Paula Gameiro (Author) FCUP; Victor de Freitas (Author) FCUP

Journal

Title: Biomolecules

Vol. 51

Final page: 1534

ISSN: 2218-273X

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

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Other information

Authenticus ID: P-00X-DGD

DOI: 10.3390/biom12101534

Abstract (EN): Biomimetic models are valuable platforms to improve our knowledge on the molecular mechanisms governing membrane-driven processes in (patho)physiological conditions, including membrane permeability, transport, and fusion. However, current membrane models are over simplistic and do not include the membrane's lipid remodelling in response to extracellular stimuli. Our study describes the synthesis of glycated dimyristoyl-phosphatidylethanolamine (DMPE-glyc), which was structurally characterised by mass spectrometry (ESI-MS) and quantified by NMR spectroscopy to be further incorporated in a complex phospholipid (PL) membrane model enriched in cholesterol (Chol) and (glyco)sphingolipids (GSL) designed to mimic epithelial membranes (PL/Chol/GSL) under hyperglycaemia conditions. Characterisation of synthesised DMPE-glyc adducts by tandem mass spectrometry (ESI-MS/MS) show that synthetic DMPE-glyc adducts correspond to Amadori products and quantification by H-1 NMR spectroscopy show that the yield of glycation reaction was 8%. The biophysical characterisation of the epithelial membrane model shows that excess glucose alters the thermotropic behaviour and fluidity of epithelial membrane models likely to impact permeability of solutes. The epithelial membrane models developed to mimic normo- and hyperglycaemic scenarios are the basis to investigate (poly)phenol-lipid and drug-membrane interactions crucial in nutrition, pharmaceutics, structural biochemistry, and medicinal chemistry.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15